A bilirubin colorimeter assay is a useful tool in evaluating bilirubin levels in patients. This blood test measures the concentration of total and conjugated bilirubin in the plasma. It is recommended to use fresh or non-hemolysis plasma samples. The frozen sample can also be used if it has not been subjected to multiple freeze-thaw cycles. The bilirubin colorimeter assay can measure total conjugated cholecalcaemia. However, this test cannot distinguish between different isoforms of bilirubin.
There are several advantages to the bilirubin colorimeter assay. First of all, it uses a simple and inexpensive paper-based method to determine the concentration of unconjugated bilirubin. The method is also sensitive to the DMSO solvent system and interacts with the sample matrix. The bilirubin colorimeter essay is an effective way to measure the amount of bilirubin in a patient's blood. In contrast to hemoglobin, bilirubin is a colorless compound. It is produced by the liver and has several biological functions. The blood of individuals suffering from the hepatic disease has a higher level of bilirubin than normal healthy people. For instance, in May 2021, a joint study carried out by the U.S. Department of Pediatrics and Makerere University College of Health Sciences (Uganda) reported the prevalence of malaria-related acute kidney injury in African children as a result of high bilirubin concentrations in their blood.
The bilirubin colorimeter assay utilizes the Jendrassik-Grof principle to determine the total bilirubin content. In the presence of the catalyst, the bilirubin is deconjugated, forming a red substance called azobilirubin. This metabolite absorbs light at 600 nm. Similarly, azobilirubin is in conjugated form. The test is highly sensitive and accurate. The results are also fast and can be used in clinical settings. The results are usually available immediately. The sample should be stored for a minimum of 24 hours and should not be refrigerated overnight. The result should be within a few days. The method can take up to four to six weeks. The CV of the enzymatic method is approximately 2.4%.
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